Reovirus reverse genetics: Incorporation of the CAT gene into the reovirus genome
نویسندگان
چکیده
منابع مشابه
Control of transcription of the reovirus genome.
The ten double-stranded RNA segments of the reovirus genome are not transcribed at equal frequencies until later times during the course of infection. When an inhibitor of protein synthesis such as cycloheximide was added to infected cells at the beginning of infection, only four of the ten segments were transcribed. By two hr post infection, five and possibly seven of the segments were being t...
متن کاملStructure and function of the reovirus genome.
INTRODUCTION ................ ................................ 483 DISCOVERY OF REOVIRUSES .............................................. 483 STRUCTURE OF THE REOVIRUS PARTICLE .................. ............. 484 REOVIRUS GENOME ................................................ 484 PROTEINS OF REOVIRUS ................................................ 486 INFORMATION CONTENT OF THE 10 REOVIRUS G...
متن کاملSpecificity in transcription of the reovirus genome.
Reovirus contains double-stranded ribonucleic acid with a molecular weight of approximately 107 daltons. 1 2 This RNA exists in the virion as a continuous linear structure, but it is extraordinarily susceptible to breakage.3-5 When isolated by any of the commonly used extraction techniques, the major portion of viral RNA appears as fragments with lengths of 0.35 ,u, 0.6 gA, and 1.1 jA, correspo...
متن کاملIdentification of signals required for the insertion of heterologous genome segments into the reovirus genome.
In cells simultaneously infected with any two of the three reovirus serotypes ST1, ST2, and ST3, up to 15% of the yields are intertypic reassortants that contain all possible combinations of parental genome segments. We have now found that not all genome segments in reassortants are wild type. In reassortants that possess more ST1 than ST3 genome segments, all ST1 genome segments appear to be w...
متن کاملAsynchronous synthesis of the complementary strands of the reovirus genome.
The mechanism of replication of the double-stranded RNA genome of reovirus has been analyzed by tracing the fate of the parental double-stranded RNA genome and by determining whether the complementary strands, which comprise the progeny double-stranded RNA, are synthesized simultaneously or sequentially. The results indicate that the parental double-stranded RNA is conserved as the original dup...
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ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 2001
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.131203198